Nuclear RNA purification by flow cytometry to study nuclear processes in plants

The nature of plant tissues has continuously hampered understanding of the spatio-temporal and subcellular distribution of RNA-guided processes. Here, we describe a universal protocol based on Arabidopsis to investigate subcellular RNA distribution from virtually any plant species using flow cytomet...

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Vydáno v:STAR Protoc
Hlavní autoři: Moro, Belén, Kisielow, Malgorzata, Borrero, Veronica Barragan, Bouet, Antoine, Brosnan, Christopher A., Bologna, Nicolás G.
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2021
Témata:
Protocol
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7890302/
https://ncbi.nlm.nih.gov/pubmed/33659901
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100320
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