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Comparison of Four Nuclear Isolation Buffers for Plant DNA Flow Cytometry

• Background and Aims DNA flow cytometry requires preparation of suspensions of intact nuclei, which are stained using a DNA-specific fluorochrome prior to analysis. Various buffer formulas were developed to preserve nuclear integrity, protect DNA from degradation and facilitate its stoichiometric s...

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Main Authors: LOUREIRO, JOÃO, RODRIGUEZ, ELEAZAR, DOLEŽEL, JAROSLAV, SANTOS, CONCEIÇÃO
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2006
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2803574/
https://ncbi.nlm.nih.gov/pubmed/16820407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/aob/mcl141
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