A Novel Bioluminescent Protease Assay Using Engineered Firefly Luciferase

Proteases play important roles in a variety of disease processes. Understanding their biological functions underpins the efforts of drug discovery. We have developed a bioluminescent protease assay using a circularly permuted form of firefly luciferase, wherein the native enzyme termini were joined...

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Bibliografiske detaljer
Main Authors: Wigdal, Susan S, Anderson, Jessica L, Vidugiris, Gediminas J, Shultz, John, Wood, Keith V, Fan, Frank
Format: Artigo
Sprog:Inglês
Udgivet: Bentham Open 2008
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2803436/
https://ncbi.nlm.nih.gov/pubmed/20161840
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2174/1875397300802010016
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