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A Novel Bioluminescent Protease Assay Using Engineered Firefly Luciferase
Proteases play important roles in a variety of disease processes. Understanding their biological functions underpins the efforts of drug discovery. We have developed a bioluminescent protease assay using a circularly permuted form of firefly luciferase, wherein the native enzyme termini were joined...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bentham Open
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2803436/ https://ncbi.nlm.nih.gov/pubmed/20161840 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2174/1875397300802010016 |
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