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Profiling the selectivity of DNA ligases in an array format with mass spectrometry
This article describes a method for the global profiling of the substrate specificities of DNA ligases and illustrates examples using the Taq and T4 DNA ligases. The method combines oligonucleotide arrays, which offer the benefits of high throughput and multiplexed assays, with mass spectrometry to...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
2010
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2800213/ https://ncbi.nlm.nih.gov/pubmed/19854942 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp827 |
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