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Profiling the selectivity of DNA ligases in an array format with mass spectrometry

This article describes a method for the global profiling of the substrate specificities of DNA ligases and illustrates examples using the Taq and T4 DNA ligases. The method combines oligonucleotide arrays, which offer the benefits of high throughput and multiplexed assays, with mass spectrometry to...

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Bibliografische gegevens
Hoofdauteurs: Kim, Joohoon, Mrksich, Milan
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2010
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2800213/
https://ncbi.nlm.nih.gov/pubmed/19854942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp827
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