Optimization of immunoprecipitation–western blot analysis in detecting GW182-associated components of GW/P bodies

Characterizing the components of GW/processing bodies is key to elucidating RNA interference and messenger RNA processing pathways. This protocol addresses challenges in isolating a low-abundance protein GW182 and GW body (GWB)-associated proteins by building on previous reports that used polyclonal...

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Hlavní autoři: Moser, Joanna J, Chan, Edward K L, Fritzler, Marvin J
Médium: Artigo
Jazyk:Inglês
Vydáno: 2009
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2797048/
https://ncbi.nlm.nih.gov/pubmed/19373232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.34
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