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Optimization of immunoprecipitation–western blot analysis in detecting GW182-associated components of GW/P bodies
Characterizing the components of GW/processing bodies is key to elucidating RNA interference and messenger RNA processing pathways. This protocol addresses challenges in isolating a low-abundance protein GW182 and GW body (GWB)-associated proteins by building on previous reports that used polyclonal...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2797048/ https://ncbi.nlm.nih.gov/pubmed/19373232 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.34 |
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