Direct sequencing of enzymatically amplified human genomic DNA.
The polymerase chain reaction is a recently described technique that uses flanking oligonucleotide primers and repeated cycles of enzymatic primer extension to amplify a short segment of DNA by greater than 100,000-fold. By use of sequencing primers located internal to the amplification primers, dir...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1988
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279587/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3267215/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.2.544 |
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