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Direct sequencing of enzymatically amplified human genomic DNA.

The polymerase chain reaction is a recently described technique that uses flanking oligonucleotide primers and repeated cycles of enzymatic primer extension to amplify a short segment of DNA by greater than 100,000-fold. By use of sequencing primers located internal to the amplification primers, dir...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Engelke, D R, Hoener, P A, Collins, F S
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279587/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3267215/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.2.544
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