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Direct sequencing of enzymatically amplified human genomic DNA.

The polymerase chain reaction is a recently described technique that uses flanking oligonucleotide primers and repeated cycles of enzymatic primer extension to amplify a short segment of DNA by greater than 100,000-fold. By use of sequencing primers located internal to the amplification primers, dir...

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Detalhes bibliográficos
Main Authors: Engelke, D R, Hoener, P A, Collins, F S
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC279587/
https://ncbi.nlm.nih.gov/pubmed/3267215
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