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Single-molecule measurements of synthesis by DNA polymerase with base-pair resolution
The catalytic mechanism of DNA polymerases involves multiple steps that precede and follow the transfer of a nucleotide to the 3′-hydroxyl of the growing DNA chain. Here we report a single-molecule approach to monitor the movement of E. coli DNA polymerase I (Klenow fragment) on a DNA template durin...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2795520/ https://ncbi.nlm.nih.gov/pubmed/19955412 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0908640106 |
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