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Chronic Reduction of the Cytosolic or Mitochondrial NAD(P)-malic Enzyme Does Not Affect Insulin Secretion in a Rat Insulinoma Cell Line

The cytosolic malic enzyme (ME1) has been suggested to augment insulin secretion via the malate-pyruvate and/or citrate-pyruvate shuttles, through the production of NADPH or other metabolites. We used selectable vectors expressing short hairpin RNA (shRNA) to stably decrease Me1 mRNA levels by 80–86...

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Autors principals: Brown, Laura J., Longacre, Melissa J., Hasan, Noaman M., Kendrick, Mindy A., Stoker, Scott W., MacDonald, Michael J.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2790965/
https://ncbi.nlm.nih.gov/pubmed/19858194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.040394
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