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Cytomegalovirus Promoter Up-regulation Is the Major Cause of Increased Protein Levels of Unstable Reporter Proteins after Treatment of Living Cells with Proteasome Inhibitors

Fluorescent unstable proteins obtained by the fusion of a fluorescent protein coding sequence with specific amino acid sequences that promote its fast degradation have become popular to gauge the activity of the ubiquitin/proteasome system in living cells. The steady-state levels of expression of th...

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Detalhes bibliográficos
Main Authors: Alvarez-Castelao, Beatriz, Martín-Guerrero, Idoia, García-Orad, África, Castaño, José G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2788877/
https://ncbi.nlm.nih.gov/pubmed/19679666
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.004101
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