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One-plasmid tunable coexpression for mycobacterial protein–protein interaction studies

A single plasmid that allows controlled coexpression has been developed for use in mycobacteria. The tetracycline inducible promoter, PtetO, was used to provide tetracycline-dependent induction of one gene, while the Psmyc, Pimyc, or Phsp promoters were used to provide three different levels of cons...

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Sonraí Bibleagrafaíochta
Main Authors: Chang, Yong, Mead, David, Dhodda, Vinay, Brumm, Phil, Fox, Brian G
Formáid: Artigo
Teanga:Inglês
Foilsithe: Wiley Subscription Services, Inc., A Wiley Company 2009
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2788286/
https://ncbi.nlm.nih.gov/pubmed/19760663
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.242
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