Spurious Amplification of a Plasmodium vivax Small-Subunit RNA Gene by Use of Primers Currently Used To Detect P. knowlesi

The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <...

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Detaylı Bibliyografya
Asıl Yazarlar: Imwong, Mallika, Tanomsing, Naowarat, Pukrittayakamee, Sasithon, Day, Nicholas P. J., White, Nicholas J., Snounou, Georges
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology (ASM) 2009
Konular:
Parasitology
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2786678/
https://ncbi.nlm.nih.gov/pubmed/19812279
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00811-09
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