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Restriction Site-dependent PCR: An Efficient Technique for Fast Cloning of New Genes of Microorganisms

New bioactive proteins need to be screened from various microorganisms for the increasing need for industrial and pharmaceutical peptide, proteins, or enzymes. A novel polymerase chain reaction (PCR) method, restriction site-dependent PCR (RSD-PCR), was designed for rapid new genes cloning from geno...

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Autori principali: Jiang, Yu, Pei, Jianjun, Song, Xin, Shao, Weilan
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2007
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2779911/
https://ncbi.nlm.nih.gov/pubmed/18086803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/dnares/dsm023
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