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Quantitative analysis of chromatin compaction in living cells using FLIM–FRET
We present a quantitative Förster resonance energy transfer (FRET)–based assay using multiphoton fluorescence lifetime imaging microscopy (FLIM) to measure chromatin compaction at the scale of nucleosomal arrays in live cells. The assay uses a human cell line coexpressing histone H2B tagged to eithe...
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| Asıl Yazarlar: | , , , , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
The Rockefeller University Press
2009
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2779238/ https://ncbi.nlm.nih.gov/pubmed/19948497 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.200907029 |
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