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Detection of covalent and non-covalent intermediates in the polymerization reaction catalyzed by a C149S–class III polyhydroxybutyrate synthase

Polyhydroxybutyrate (PHB) synthases catalyze the conversion of 3-hydroxybutyryl coenzyme A (HBCoA) to PHB of molecular weight 1.5 million. The class III synthase from Allochromatium vinosum is a tetramer of PhaEPhaC (each 40 KDa). The polymerization involves covalent catalysis using C149 of PhaC wit...

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Detalhes bibliográficos
Main Authors: Li, Ping, Chakraborty, Sumit, Stubbe, JoAnne
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2777943/
https://ncbi.nlm.nih.gov/pubmed/19711985
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi901329b
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