লোডিং...
Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides
Here it is demonstrated that the yeast Saccharomyces cerevisiae can take up and assemble at least 38 overlapping single-stranded oligonucleotides and a linear double-stranded vector in one transformation event. These oligonucleotides can overlap by as few as 20 bp, and can be as long as 200 nucleoti...
সংরক্ষণ করুন:
| প্রধান লেখক: | |
|---|---|
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
Oxford University Press
2009
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2777417/ https://ncbi.nlm.nih.gov/pubmed/19745056 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp687 |
| ট্যাগগুলো: |
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