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Method of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments
In the era of synthetic biology, techniques for rapidly constructing a designer long DNA from short DNA fragments are desired. To realize this, we attempted to establish a method for one-step DNA assembly of unprecedentedly large numbers of fragments. The basic technology is the Ordered Gene Assembl...
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| Pubblicato in: | Sci Rep |
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| Autori principali: | , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Nature Publishing Group
2015
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4438487/ https://ncbi.nlm.nih.gov/pubmed/25990947 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep10655 |
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