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One step assembly of multiple DNA fragments with a designed order and orientation in Bacillus subtilis plasmid

A universal method to reconstitute sets of genes was developed. Owing to the intrinsic nature of the plasmid establishment mechanism in Bacillus subtilis, the assembly of five antibiotic resistance genes with a defined order and orientation was achieved. These five fragments and the plasmid have thr...

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Bibliografiske detaljer
Main Authors: Tsuge, Kenji, Matsui, Kuniko, Itaya, Mitsuhiro
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2003
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC275490/
https://ncbi.nlm.nih.gov/pubmed/14576333
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng133
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