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One step assembly of multiple DNA fragments with a designed order and orientation in Bacillus subtilis plasmid
A universal method to reconstitute sets of genes was developed. Owing to the intrinsic nature of the plasmid establishment mechanism in Bacillus subtilis, the assembly of five antibiotic resistance genes with a defined order and orientation was achieved. These five fragments and the plasmid have thr...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC275490/ https://ncbi.nlm.nih.gov/pubmed/14576333 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng133 |
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