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Purification, crystallization and preliminary X-ray analysis of bifunctional isocitrate dehydrogenase kinase/phosphatase in complex with its substrate, isocitrate dehydrogenase, from Escherichia coli

Escherichia coli isocitrate dehydrogenase (ICDH) can be phosphorylated and dephosphorylated by a single bifunctional protein, isocitrate dehydrogenase kinase/phosphatase (AceK), which is encoded by the aceK gene. In order to investigate the regulatory mechanism of (de)phosphorylation of ICDH by AceK...

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Bibliographic Details
Main Authors: Zheng, Jimin, Ji, Alan Xian, Jia, Zongchao
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2009
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2777047/
https://ncbi.nlm.nih.gov/pubmed/19923739
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109038718
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