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Detection of early unfolding events in a dimeric protein by amide proton exchange and native electrospray mass spectrometry
Oligomeric proteins generally undergo unfolding through a dissociation/denaturation mechanism wherein the subunits first dissociate and then unfold. This mechanism can be detected by the fact that the proteins exhibit a concentration dependence of the denaturation curve. However, the concentration d...
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| Principais autores: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Wiley Subscription Services, Inc., A Wiley Company
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2776950/ https://ncbi.nlm.nih.gov/pubmed/19554628 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.176 |
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