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Determining protein stability in cell lysates by pulse proteolysis and Western blotting

Proteins require proper conformational energetics to fold and to function correctly. Despite the importance of having information on conformational energetics, the investigation of thermodynamic stability has been limited to proteins, which can be easily expressed and purified. Many biologically imp...

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Detalhes bibliográficos
Main Authors: Kim, Moon-Soo, Song, Jiao, Park, Chiwook
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2771307/
https://ncbi.nlm.nih.gov/pubmed/19388050
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.115
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