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Protocol for determining protein cysteine thiol redox status using western blot analysis
This protocol describes the analysis of protein cysteine redox status. Redox status is crucial in regulating protein activity, stability, and redox signaling cascades. It is determined by conjugation with 1.24 kDa MM(PEG)(24) molecule to each reduced cysteine followed by western blot analysis. This...
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| Publié dans: | STAR Protoc |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Elsevier
2021
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8196218/ https://ncbi.nlm.nih.gov/pubmed/34159320 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100566 |
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