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Determining Biophysical Protein Stability in Lysates by a Fast Proteolysis Assay, FASTpp
The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp). Combining unfolding by a temperature gradient in a thermal cycler with simultaneo...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3463568/ https://ncbi.nlm.nih.gov/pubmed/23056252 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0046147 |
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