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Rational design of a fusion partner for membrane protein expression in E. coli
We have designed a novel protein fusion partner (P8CBD) to utilize the co-translational SRP pathway in order to target heterologous proteins to the E. coli inner membrane. SRP-dependence was demonstrated by analyzing the membrane translocation of P8CBD-PhoA fusion proteins in wt and SRP-ffh77 mutant...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Wiley Subscription Services, Inc., A Wiley Company
2009
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2771104/ https://ncbi.nlm.nih.gov/pubmed/19530231 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.189 |
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