Rational design of a fusion partner for membrane protein expression in E. coli

We have designed a novel protein fusion partner (P8CBD) to utilize the co-translational SRP pathway in order to target heterologous proteins to the E. coli inner membrane. SRP-dependence was demonstrated by analyzing the membrane translocation of P8CBD-PhoA fusion proteins in wt and SRP-ffh77 mutant...

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Bibliographic Details
Main Authors: Luo, Jianying, Choulet, Julie, Samuelson, James C
Format: Artigo
Language:Inglês
Published: Wiley Subscription Services, Inc., A Wiley Company 2009
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2771104/
https://ncbi.nlm.nih.gov/pubmed/19530231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.189
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