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High-yield membrane protein expression from E. coli using an engineered outer membrane protein F fusion
Obtaining high yields of membrane proteins necessary to perform detailed structural study is difficult due to poor solubility and variability in yields from heterologous expression systems. To address this issue, an Escherichia coli-based membrane protein overexpression system utilizing an engineere...
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| Autores principales: | , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Wiley Subscription Services, Inc., A Wiley Company
2013
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3610049/ https://ncbi.nlm.nih.gov/pubmed/23345122 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2224 |
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