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High-yield membrane protein expression from E. coli using an engineered outer membrane protein F fusion

Obtaining high yields of membrane proteins necessary to perform detailed structural study is difficult due to poor solubility and variability in yields from heterologous expression systems. To address this issue, an Escherichia coli-based membrane protein overexpression system utilizing an engineere...

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Bibliografiska uppgifter
Huvudupphovsmän: Su, Pin-Chuan, Si, William, Baker, Deidre L, Berger, Bryan W
Materialtyp: Artigo
Språk:Inglês
Publicerad: Wiley Subscription Services, Inc., A Wiley Company 2013
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3610049/
https://ncbi.nlm.nih.gov/pubmed/23345122
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2224
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