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The Cys154→Gly Mutation in LacY Causes Constitutive Opening of the Hydrophilic Periplasmic Pathway
The lactose permease of Escherichia coli (LacY) is a highly dynamic membrane transport protein, while the Cys154→Gly mutant is crippled conformationally. The mutant binds sugar with high affinity, but catalyzes very little translocation across the membrane. In order to further investigate the defect...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2769995/ https://ncbi.nlm.nih.gov/pubmed/18485365 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2008.04.015 |
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