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Cloning human herpes virus 6A genome into bacterial artificial chromosomes and study of DNA replication intermediates

Cloning of large viral genomes into bacterial artificial chromosomes (BACs) facilitates analyses of viral functions and molecular mutagenesis. Previous derivations of viral BACs involved laborious recombinations within infected cells. We describe a single-step production of viral BACs by direct clon...

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Bibliografske podrobnosti
Main Authors: Borenstein, Ronen, Frenkel, Niza
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2009
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2767366/
https://ncbi.nlm.nih.gov/pubmed/19858479
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0908504106
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