Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome

A strategy for cloning and mutagenesis of an infectious herpesvirus genome is described. The mouse cytomegalovirus genome was cloned and maintained as a 230 kb bacterial artificial chromosome (BAC) in E. coli. Transfection of the BAC plasmid into eukaryotic cells led to a productive virus infection....

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Autores principales: Messerle, Martin, Crnkovic, Irena, Hammerschmidt, Wolfgang, Ziegler, Heike, Koszinowski, Ulrich H.
Formato: Artigo
Lenguaje:Inglês
Publicado: The National Academy of Sciences of the USA 1997
Materias:
Biological Sciences
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC25110/
https://ncbi.nlm.nih.gov/pubmed/9405686
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