Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome

A strategy for cloning and mutagenesis of an infectious herpesvirus genome is described. The mouse cytomegalovirus genome was cloned and maintained as a 230 kb bacterial artificial chromosome (BAC) in E. coli. Transfection of the BAC plasmid into eukaryotic cells led to a productive virus infection....

Полное описание

Библиографические подробности

Опубликовано в::	Proc Natl Acad Sci U S A
Главные авторы:	Messerle, Martin, Crnkovic, Irena, Hammerschmidt, Wolfgang, Ziegler, Heike, Koszinowski, Ulrich H.
Формат:	Artigo
Язык:	Inglês
Опубликовано:	National Academy of Sciences 1997
Предметы:	Biological Sciences
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC25110/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9405686/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.94.26.14759
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