Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome
A strategy for cloning and mutagenesis of an infectious herpesvirus genome is described. The mouse cytomegalovirus genome was cloned and maintained as a 230 kb bacterial artificial chromosome (BAC) in E. coli. Transfection of the BAC plasmid into eukaryotic cells led to a productive virus infection....
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC25110/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9405686/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.94.26.14759 |
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