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Cloning human herpes virus 6A genome into bacterial artificial chromosomes and study of DNA replication intermediates

Cloning of large viral genomes into bacterial artificial chromosomes (BACs) facilitates analyses of viral functions and molecular mutagenesis. Previous derivations of viral BACs involved laborious recombinations within infected cells. We describe a single-step production of viral BACs by direct clon...

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Bibliographic Details
Main Authors: Borenstein, Ronen, Frenkel, Niza
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2009
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2767366/
https://ncbi.nlm.nih.gov/pubmed/19858479
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0908504106
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