Modulation of Single-Cell IgG Secretion Frequency and Rates in Human Memory B Cells by CpG DNA, CD40L, IL-21 and Cell Division

During the recall response by CD27+ IgG class switched human memory B cells, total IgG secreted is a function of (1) the number of IgG secreting cells (IgG-SC) and (2) the secretion rate of each cell. Here we report the quantitative ELISPOT method (qELISPOT) for simultaneous estimation of single cel...

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Detalhes bibliográficos
Main Authors: Henn, Alicia D., Rebhahn, Jonathan, Brown, Miguel A., Murphy, Alison J., Coca, Mircea N., Hyrien, Ollivier, Pellegrin, Tina, Mosmann, Tim, Zand, Martin S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2765874/
https://ncbi.nlm.nih.gov/pubmed/19675172
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4049/jimmunol.0804233
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