Real-time fluorescence detection of exoribonucleases

The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic RNA–DNA hybrid is a powerful tool for a real-time fluorescence detection and assay of exoribonucleases (R...

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Détails bibliographiques
Auteurs principaux: Sinturel, Flore, Pellegrini, Olivier, Xiang, Song, Tong, Liang, Condon, Ciarán, Bénard, Lionel
Format: Artigo
Langue:Inglês
Publié: Cold Spring Harbor Laboratory Press 2009
Sujets:
Method
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2764478/
https://ncbi.nlm.nih.gov/pubmed/19767421
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1670909
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