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Real-time detection of DNA topological changes with a fluorescently labeled cruciform

Topoisomerases are essential cellular enzymes that maintain the appropriate topological status of DNA and are the targets of several antibiotic and chemotherapeutic agents. High-throughput (HT) analysis is desirable to identify new topoisomerase inhibitors, but standard in vitro assays for DNA topol...

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Detalhes bibliográficos
Main Authors: Jude, Kevin M., Hartland, Abbey, Berger, James M.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3711437/
https://ncbi.nlm.nih.gov/pubmed/23680786
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt413
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