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Spectral and Kinetic Characterization of the Michaelis Charge Transfer Complex in Monomeric Sarcosine Oxidase

Monomeric sarcosine oxidase is a flavoenzyme that catalyzes the oxidation of the methyl group in sarcosine (N-methylglycine). Rapid reaction kinetic studies under anaerobic conditions at pH 8.0 show that the enzyme forms a charge transfer Michaelis complex with sarcosine (E-FAD(ox)·sarcosine) that e...

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Hlavní autoři: Zhao, Gouhua, Jorns, Marilyn Schuman
Médium: Artigo
Jazyk:Inglês
Vydáno: 2006
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2764459/
https://ncbi.nlm.nih.gov/pubmed/16681370
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi0600852
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