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Biosynthesis of Covalently Bound Flavin: Isolation and In Vitro Flavinylation of Monomeric Sarcosine Oxidase Apoprotein
The covalently bound FAD in native monomeric sarcosine oxidase (MSOX) is attached to the protein by a thioether bond between the 8α-methyl group of the flavin and Cys315. Large amounts of soluble apoenzyme are produced by controlled expression in a riboflavin-dependent E. coli strain. A time-depende...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2005
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1993914/ https://ncbi.nlm.nih.gov/pubmed/15850379 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi047271x |
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