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Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

An essential tool for investigating the role of a gene during development is the ability to perform gene knockdown, overexpression, and misexpression studies. In zebrafish (Danio rerio), microinjection of RNA, DNA, proteins, antisense oligonucleotides and other small molecules into the developing em...

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Bibliografiske detaljer
Main Authors: Yuan, Shiaulou, Sun, Zhaoxia
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2762915/
https://ncbi.nlm.nih.gov/pubmed/19488022
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1113
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