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Microinjection of Zebrafish Embryos to Analyze Gene Function

One of the advantages of studying zebrafish is the ease and speed of manipulating protein levels in the embryo. Morpholinos, which are synthetic oligonucleotides with antisense complementarity to target RNAs, can be added to the embryo to reduce the expression of a particular gene product. Conversel...

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Détails bibliographiques
Auteurs principaux: Rosen, Jonathan N., Sweeney, Michael F., Mably, John D.
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2009
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2762901/
https://ncbi.nlm.nih.gov/pubmed/19274045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1115
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