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Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo

Microtubules (MTs) are dynamic and fragile structures that are challenging to image in vivo, particularly in vertebrate embryos. Immunolabeling methods are described here to analyze distinct populations of MTs in the developing neural tube of the zebrafish embryo. While the focus is on neural tissue...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: McFarland, Rebecca J., Brown, Sharlene P., Vital, Eudorah, Werner, Jonathan M., Brewster, Rachel M.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5752298/
https://ncbi.nlm.nih.gov/pubmed/28994750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55792
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