Separation of 1–23-kb complementary DNA strands by urea–agarose gel electrophoresis

Double-stranded (ds), as well as denatured, single-stranded (ss) DNA samples can be analyzed on urea–agarose gels. Here we report that after denaturation by heat in the presence of 8 M urea, the two strands of the same ds DNA fragment of ∼1–20-kb size migrate differently in 1 M urea containing agaro...

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Bibliographic Details
Main Authors: Hegedüs, Éva, Kókai, Endre, Kotlyar, Alexander, Dombrádi, Viktor, Szabó, Gábor
Format: Artigo
Language:Inglês
Published: Oxford University Press 2009
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2761254/
https://ncbi.nlm.nih.gov/pubmed/19553189
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp539
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