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Separation of 1–23-kb complementary DNA strands by urea–agarose gel electrophoresis
Double-stranded (ds), as well as denatured, single-stranded (ss) DNA samples can be analyzed on urea–agarose gels. Here we report that after denaturation by heat in the presence of 8 M urea, the two strands of the same ds DNA fragment of ∼1–20-kb size migrate differently in 1 M urea containing agaro...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
2009
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2761254/ https://ncbi.nlm.nih.gov/pubmed/19553189 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp539 |
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