Differences between Ca(2+) and Mg(2+) in DNA binding and release by the SfiI restriction endonuclease: implications for DNA looping

Many enzymes acting on DNA require Mg(2+) ions not only for catalysis but also to bind DNA. Binding studies often employ Ca(2+) as a substitute for Mg(2+), to promote DNA binding whilst disallowing catalysis. The SfiI endonuclease requires divalent metal ions to bind DNA but, in contrast to many sys...

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Main Authors: Bellamy, Stuart R. W., Kovacheva, Yana S., Zulkipli, Ishan Haji, Halford, Stephen E.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2009
Assuntos:
Nucleic Acid Enzymes
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2760798/
https://ncbi.nlm.nih.gov/pubmed/19596810
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp569
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