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Differences between Ca(2+) and Mg(2+) in DNA binding and release by the SfiI restriction endonuclease: implications for DNA looping
Many enzymes acting on DNA require Mg(2+) ions not only for catalysis but also to bind DNA. Binding studies often employ Ca(2+) as a substitute for Mg(2+), to promote DNA binding whilst disallowing catalysis. The SfiI endonuclease requires divalent metal ions to bind DNA but, in contrast to many sys...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
2009
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2760798/ https://ncbi.nlm.nih.gov/pubmed/19596810 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp569 |
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