Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells

Fluorescein-labeled oligodeoxynucleotides (oligos) were introduced into cultured rat myoblasts, and their molecular movements inside the nucleus were studied by fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP). FCS revealed that a large fraction of bo...

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izdano v:	Proc Natl Acad Sci U S A
Principais autores:	Politz, Joan C., Browne, Elizabeth S., Wolf, David E., Pederson, Thoru
Format:	Artigo
Jezik:	Inglês
Izdano:	National Academy of Sciences 1998
Teme:	Biological Sciences
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC27582/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9600914/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.11.6043
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