Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells

Fluorescein-labeled oligodeoxynucleotides (oligos) were introduced into cultured rat myoblasts, and their molecular movements inside the nucleus were studied by fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP). FCS revealed that a large fraction of bo...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Politz, Joan C., Browne, Elizabeth S., Wolf, David E., Pederson, Thoru
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1998
Soggetti:	Biological Sciences
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC27582/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9600914/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.11.6043
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