Rapid Purification and High Sensitivity Analysis of Heparan Sulfate from Cells and Tissues: TOWARD GLYCOMICS PROFILING

Studies on glycosaminoglycans and proteoglycans (PGs) have been hampered by difficulties in isolation and analysis by traditional methods that are laborious and lack sensitivity and throughput. Here we demonstrate a simple method for rapid isolation of proteoglycans (RIP) employing phenol/guanidine/...

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Hlavní autoři: Guimond, Scott E., Puvirajesinghe, Tania M., Skidmore, Mark A., Kalus, Ina, Dierks, Thomas, Yates, Edwin A., Turnbull, Jeremy E.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Biochemistry and Molecular Biology 2009
Témata:
Glycobiology and Extracellular Matrices
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2757973/
https://ncbi.nlm.nih.gov/pubmed/19596853
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.032755
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